Plasmid

Part:BBa_K4761106

Designed by: Leidun Xu   Group: iGEM23_HiZJU-China   (2023-09-29)


pET28a

pET28a expression vector for new RNA Thermometer units. Note that it has no antibiotics resistance.

This vector is obtained from pET28a-EGFP, and later was used to be the vector of RNA Thermometer report units(BBa_K4761235 to BBa_K4761239).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5102
    Illegal BamHI site found at 5305
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5168
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 3322
    Illegal NgoMIV site found at 3482
    Illegal NgoMIV site found at 5070
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Expression activity in BL21(DE3) chassis has been confirmed in RNA Thermometer Module in 2023 HiZJU-China. See results of BBa_K4761112.

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Categories
Parameters
None